Edición especial COVID-19 Abril-Diciembre 2020 82
ISSN 1317-987X


COVID-19 diagnosis —A review of current methods
Fecha de recepción: 05/06/2020
Fecha de aceptación:


A fast and accurate self-testing tool for COVID-19 diagnosis has become a prerequisite to comprehend the exact number of cases worldwide and to take medical and governmental actions accordingly. SARS-CoV-2 (formerly, 2019-nCoV) infection was first reported in Wuhan (China) in December 2019, and then it has rapidly spread around the world, causing ~14 million active cases with ~582,000 deaths as of July 2020. The diagnosis tools available so far have been based on a) viral gene detection, b) human antibody detection, and c) viral antigen detection, among which the viral gene detection by RT-PCR has been found as the most reliable technique. In this report, the current SARS-CoV-2 detection kits, exclusively the ones that were issued an “Emergency Use Authorization” from the U.S. Food and Drug Administration, were discussed. The key structural components of the virus were presented to provide the audience with an understanding of the scientific principles behind the testing tools. The methods that are still in the early research state were also reviewed in a subsection based on the reports available so far.

Key Word
COVID-19 SARS-CoV-2 diagnosis SARS-CoV-2 detection RT-PCR Lateral flow assay Loop-mediated isothermal amplification Point of care devices

COVID-19 diagnosis —A review of current methods

Meral Yüce a,*, Elif Filiztekin b, Korin Gasia ¨Ozkaya b

a SUNUM Nanotechnology Research and Application Centre, Sabanci University, 34956, Orhanli, Tuzla, Istanbul, Turkey

b Faculty of Engineering and Natural Sciences and, Sabanci University, 34956, Orhanli, Tuzla, Istanbul, Turkey

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, thecompany's public news and information website.

Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

COVID-19 diagnosis —A review of current methods

NOTA: Toda la información que se brinda en este artículo es de carácter investigativo y con fines académicos y de actualización para estudiantes y profesionales de la salud. En ningún caso es de carácter general ni sustituye el asesoramiento de un médico. Ante cualquier duda que pueda tener sobre su estado de salud, consulte con su médico o especialista.

Instituto de Medicina Tropical - Facultad de Medicina - Universidad Central de Venezuela.
Elaborado por el Centro de Análisis de Imágenes Biomédicas Computarizadas CAIBCO,
Este portal ha sido desarrollado gracias al apoyo del Fonacit